Actinorhodin production by Streptomyces coelicolor A3(2): kinetic parameters related to growth, substrate uptake and production
Applied Microbiology and Biotechnology, cilt.40, sa.4, ss.457-462, 1993 (Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 40 Sayı: 4
- Basım Tarihi: 1993
- Doi Numarası: 10.1007/bf00175730
- Dergi Adı: Applied Microbiology and Biotechnology
- Derginin Tarandığı İndeksler: Scopus
- Sayfa Sayıları: ss.457-462
- Boğaziçi Üniversitesi Adresli: Evet
Özet
The dynamics of an Streptomyces coelicolor A3(2) culture in a 20-l computer-controlled batch bioreactor was investigated both experimentally and theoretically. In defined medium, depending on the initial conditions, the calculated value of some of the kinetic parameters were: maximum specific growth rate, 0.03 h-1; death rate constant, 1.4-6.3 × 10-3 h-1; observed biomass yield, 0.21 g cells g-1 glucose and the maintenance coefficient for the cells, 0.0448 g glucose g-1 cells h-1. According to both experimental observations and the Luedeking-Piret model, actinorhodin production was found to be growth-associated. This paper provides the first published quantitative information on the main kinetic parameters describing the activity of S. coelicolor in batch culture. © 1993 Springer-Verlag.