Optimization of the mobile phase conditions for the purification of Taql endonuclease
Chromatographia, cilt.52, sa.5-6, ss.377-380, 2000 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 52 Sayı: 5-6
- Basım Tarihi: 2000
- Doi Numarası: 10.1007/bf02491037
- Dergi Adı: Chromatographia
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.377-380
- Anahtar Kelimeler: Column liquid chromatography, Ion exchange chromatography, Taql endonuclease
- Boğaziçi Üniversitesi Adresli: Evet
Özet
Taql endonuclease was purified by high performance ion exchange liquid chromatography with linear gradient elution. Of the chromatographic media tested as mobile phases for the HPLC purification of Taql endonuclease, sodium acetate (pH 5.0) and phosphate (pH 7.0) buffers were appropriate for use with cation exchange columns, and L-histidine (pH 6.0) and Tris (pH 8.0) buffers with anion exchange columns.