Whole genome sequencing of Thametopoea pityocampa revealed putative pesticide targets
Genomics, cilt.112, sa.6, ss.4203-4207, 2020 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 112 Sayı: 6
- Basım Tarihi: 2020
- Doi Numarası: 10.1016/j.ygeno.2020.07.017
- Dergi Adı: Genomics
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS, CAB Abstracts, Chemical Abstracts Core, EMBASE, MEDLINE, Veterinary Science Database
- Sayfa Sayıları: ss.4203-4207
- Anahtar Kelimeler: G-protein coupled receptors, Neuropeptides, Next-generation pesticide, Transcription, Whole genome sequencing
- Boğaziçi Üniversitesi Adresli: Evet
Özet
Insect neuropeptides play a major role in the regulation of the physiological processes. Due to their versatile effects on the development of insects, their corresponding receptors, which are mostly G-protein coupled receptors, are considered as ideal targets for designing next-generation pesticides. In this study, we aimed to find neuropeptide receptors of pine processionary moth (Thaumetopoea pityocampa), a pest in the Mediterranean countries, that feeds on the needles of pine trees. To this aim, Whole Genome Shotgun sequencing technique was used. de novo assembly of the genome was performed using two different assemblers, SGA and MaSuRCA. The results of two assemblers were compared, and MaSuRCA assembler showed higher N50 length. To find some target GPCRs, sequences of Drosophila melanogaster and evolutionarily close species were used as blast queries in the assembled data. Five GPCRs were chosen from the genome and their expression was confirmed in the larval stage of the insect.