Cybernetic modelling of growth and ethanol production in a recombinant Saccharomyces cerevisiae strain secreting a bifunctional fusion protein
Process Biochemistry, cilt.37, sa.12, ss.1439-1445, 2002 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 37 Sayı: 12
- Basım Tarihi: 2002
- Doi Numarası: 10.1016/s0032-9592(02)00031-6
- Dergi Adı: Process Biochemistry
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.1439-1445
- Anahtar Kelimeler: Bifunctional fusion protein, Cybernetic modelling, Recombinant yeast, Starch bioconversion, Substitutable substrates
- Boğaziçi Üniversitesi Adresli: Evet
Özet
Recombinant Saccharomyces cerevisiae YPB-G strain secreting a fusion protein displaying both BsAAase/GAase activities was grown in 1.5 1 YPS media containing single (starch) and mixed carbon sources (glucose+starch) using a 2.5 1 New Brunswick BiofloIII fermenter. Ethanol and biomass formation, starch utilisation, secretion of the amylolytic enzymes (α-amylase and glucoamylase), accumulation of reducing sugars and glucose were followed during the fermentation of YPB-G under different conditions. Moreover, a model has been developed for the growth of recombinant yeast on substitutable substrates using cybernetic framework principles and incorporating product formation. In the present work, both the biphasic and the diauxic growth patterns observed experimentally in batch culture of recombinant yeast cells were simulated successfully by modifying the cybernetic framework to include ethanol formation and the degradation kinetics of starch which is not directly utilised by yeast. The model can further be expanded to fed-batch systems. © 2002 Elsevier Science Ltd. All rights reserved.